SY-03B-DK Syringe Pump Accuracy Test: 4 uL Micro-Dispensing Results

Introduction

SY-03B-DK syringe pump accuracy test data are useful when engineers need to evaluate microliter-scale dispensing under a defined fluid path, syringe size, speed setting, and outlet restriction. In this test, the target dispense volume was 4 uL using a 500 uL borosilicate glass syringe, deionized water, and a micro-needle outlet with 0.4 mm inner diameter.

The objective was to evaluate real dispensing behavior under a customer-style micro-volume condition, not to claim a universal accuracy value for every liquid or system. The results should be read as application test data under the listed setup: SY-03B-DK-60-T6-F-PEEK, RS232 control through SerialComm V1.3.0, V6000 speed setting, and gravimetric measurement over 10 consecutive runs.

Quick Answer

In this SY-03B-DK syringe pump accuracy test, the target dispense volume was 4 uL, equivalent to 0.0040 g when using deionized water and an approximate water density of 1 g/cm3. Across 10 measured runs, the average dispensed mass was 0.00402 g, approximately 4.02 uL. The reported coefficient of variation was 5.84%, and the maximum single deviation was 0.0006 g, or approximately 0.6 uL.

The data show that the average output was close to the 4 uL target under the documented test conditions. Repeatability was affected by one higher run at 0.0046 g, which should be considered when evaluating micro-volume dispensing systems where bubbles, backpressure, outlet restriction, and conditioning steps can influence results.

Test Setup for the SY-03B-DK Syringe Pump Accuracy Test

The test used a defined hardware and fluidic configuration. Because microliter dispensing is sensitive to outlet geometry, trapped air, tubing condition, and liquid properties, the setup should be stated clearly before interpreting the results.

Figure 1. Test setup used for the 4 uL micro-dispensing evaluation.

Measurement Method: Gravimetric Micro-Dispensing

The test used gravimetric measurement. The fluid path, including the syringe and tubing, was pre-filled with deionized water to reduce trapped air. The syringe aspirated a full 500 uL volume, then dispensed 4 uL per run through the micro-needle outlet. Each dispensed sample was weighed on an analytical balance and recorded in grams.

For water-based measurement, 0.0040 g is treated as approximately 4 uL using a density approximation of 1 g/cm3. This conversion is appropriate for interpreting the test but should not be applied directly to liquids with different density without correction.

Raw Data from 10 Dispense Runs

Figure 2. Gravimetric results from 10 consecutive 4 uL dispensing runs.

Performance Summary

How to Interpret the 4 uL Accuracy Data

The average value of 4.02 uL indicates that the system output was close to the target volume under the stated conditions. This is important because micro-volume dispensing can be strongly affected by liquid density, outlet restriction, air bubbles, and fluid-path compliance.

The 5.84% CV should be interpreted in context. A 4 uL target volume is small, and the test used a restrictive 0.4 mm I.D. micro-needle at V6000. Under these conditions, one transient event can affect the calculated repeatability. Run 6 measured 0.0046 g, which was the largest deviation in the data set.

This does not mean that all applications will produce the same CV. Larger dispense volumes, different outlet needles, lower speed settings, different liquids, or improved pre-run conditioning may change repeatability. Data not available in source documents for a universal correction factor.

Practical Engineering Notes for Micro-Dispensing

- Prime the syringe, tubing, and needle to reduce trapped air before micro-volume testing.

- Verify liquid density if converting gravimetric mass to volume for non-water liquids.

- Keep tubing, fittings, and outlet needle geometry consistent between validation and production use.

- Consider pre-run conditioning when measuring very small volumes.

- Record speed setting, syringe size, outlet restriction, and environmental conditions with the result.

- Treat short-run data as an engineering evaluation, not a universal performance specification.

Comparison Table: Micro-Volume Test Conditions vs Typical Larger-Volume Dispensing

FAQ

What was the target volume in this SY-03B-DK syringe pump accuracy test?

The target dispense volume was 4 uL, measured as approximately 0.0040 g using deionized water.

What syringe was used?

The test used a 500 uL high-borosilicate glass syringe.

What was the average measured output?

The average measured mass was 0.00402 g, approximately 4.02 uL.

What was the repeatability result?

The reported coefficient of variation was 5.84% for the 10-run test under the documented setup.

Why was run 6 important?

Run 6 measured 0.0046 g, which was the largest single deviation from the 0.0040 g target and influenced the repeatability calculation.

Can these results be applied to other liquids?

Not directly. Other liquids may have different density, viscosity, surface tension, and bubble behavior. Validation should be repeated with the actual liquid.

Does this test define a universal SY-03B-DK accuracy specification?

No. It is an application-style test under specific hardware, liquid, speed, and outlet conditions.

How should engineers validate their own micro-dispensing system?

Use the actual syringe, tubing, outlet, liquid, speed, and control sequence, then measure multiple runs with a suitable balance or validated measurement method.

Conclusion

This SY-03B-DK syringe pump accuracy test provides practical data for 4 uL micro-dispensing using a 500 uL glass syringe, deionized water, a restrictive 0.4 mm I.D. micro-needle, and V6000 speed setting. The 10-run average was 0.00402 g, approximately 4.02 uL, with a reported CV of 5.84% and a maximum single deviation of 0.0006 g.

For OEM engineers, the main value of this test is not a universal claim, but a clear example of how to document micro-dispensing performance: define the fluid path, state the measurement method, show raw data, and validate under actual operating conditions.